Precise Coating of a Wide Range of DNA Templates by a Protein Polymer with a DNA Binding Domain.
作者： Armando Hernandez-Garcia , Nicole A. Estrich , Marc W. T. Werten , Johan R. C. Van Der Maarel , Thomas H. LaBean
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摘要: Emerging DNA-based nanotechnologies would benefit from the ability to modulate properties (e.g., solubility, melting temperature, chemical stability) of diverse DNA templates (single molecules or origami nanostructures) through controlled, self-assembling coatings. We here introduce a coating agent, called C8–BSso7d, which binds and coats with high specificity affinity, individual as well folded nanostructures. C8–BSso7d protects without condensing, collapsing destroying spatial structure underlying template. combines specific nonelectrostatic binding affinity an archeal-derived domain (Sso7d, 7 kDa) long hydrophilic random coil polypeptide (C8, 73 kDa), provides colloidal stability (solubility) formation polymer brushes around templates. is produced recombinantly in yeast has precise (but engineerable) amino acid sequence length. Using electrophor...
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Stephen P Edmondson, John W Shriver, DNA binding proteins Sac7d and Sso7d from Sulfolobus. Methods in Enzymology. ,vol. 334, pp. 129- 145 ,(2001) , 10.1016/S0076-6879(01)34463-4
Howard Robinson, Yi-Gui Gao, Bradford S. McCrary, Stephen P. Edmondson, John W. Shriver, Andrew H.-J. Wang, The hyperthermophile chromosomal protein Sac7d sharply kinks DNA Nature. ,vol. 392, pp. 202- 205 ,(1998) , 10.1038/32455
Hemant Kumar, Yves Lansac, Matthew A. Glaser, Prabal K. Maiti, Biopolymers in nanopores: challenges and opportunities Soft Matter. ,vol. 7, pp. 5898- 5907 ,(2011) , 10.1039/C0SM01517B
A Bensimon, A Simon, A Chiffaudel, V Croquette, F Heslot, D Bensimon, Alignment and sensitive detection of DNA by a moving interface Science. ,vol. 265, pp. 2096- 2098 ,(1994) , 10.1126/SCIENCE.7522347
Shawn M. Douglas, Hendrik Dietz, Tim Liedl, Björn Högberg, Franziska Graf, William M. Shih, Self-assembly of DNA into nanoscale three-dimensional shapes Nature. ,vol. 459, pp. 414- 418 ,(2009) , 10.1038/NATURE08016
Yi-Gui Gao, Shao-Yu Su, Howard Robinson, Savita Padmanabhan, Louis Lim, Bradford S. McCrary, Stephen P. Edmondson, John W. Shriver, Andrew H.-J. Wang, The crystal structure of the hyperthermophile chromosomal protein Sso7d bound to DNA Nature Structural & Molecular Biology. ,vol. 5, pp. 782- 786 ,(1998) , 10.1038/1822
Adam R. Hall, Stijn van Dorp, Serge G. Lemay, Cees Dekker, Electrophoretic Force on a Protein-Coated DNA Molecule in a Solid-State Nanopore Nano Letters. ,vol. 9, pp. 4441- 4445 ,(2009) , 10.1021/NL9027318
Roberto Consonni, Ivana Arosio, Barbara Belloni, Federico Fogolari, Paola Fusi, Erlet Shehi, Lucia Zetta, None, Investigations of Sso7d catalytic residues by NMR titration shifts and electrostatic calculations. Biochemistry. ,vol. 42, pp. 1421- 1429 ,(2003) , 10.1021/BI0265168
Ernest T Lam, Alex Hastie, Chin Lin, Dean Ehrlich, Somes K Das, Michael D Austin, Paru Deshpande, Han Cao, Niranjan Nagarajan, Ming Xiao, Pui-Yan Kwok, Genome mapping on nanochannel arrays for structural variation analysis and sequence assembly Nature Biotechnology. ,vol. 30, pp. 771- 776 ,(2012) , 10.1038/NBT.2303
Armando Hernandez-Garcia, Marc W. T. Werten, Martien Cohen Stuart, Frits A. de Wolf, Renko de Vries, Coating of Single DNA Molecules by Genetically Engineered Protein Diblock Copolymers Small. ,vol. 8, pp. 3491- 3501 ,(2012) , 10.1002/SMLL.201200939