A fast method using a new hydrophilic-lipophilic balanced sorbent in combination with ultra-high performance liquid chromatography for quantification of significant bioactive metabolites in wines.

摘要: Abstract This manuscript describes the development and validation of an ultra-fast, efficient, high throughput analytical method based on ultra-high performance liquid chromatography (UHPLC) equipped with a photodiode array (PDA) detection system, for simultaneous analysis fifteen bioactive metabolites: gallic acid, protocatechuic (−)-catechin, gentisic (−)-epicatechin, syringic p -coumaric ferulic m rutin, trans -resveratrol, myricetin, quercetin, cinnamic acid kaempferol, in wines. A 50-mm column packed 1.7-μm particles operating at elevated pressure (UHPLC strategy) was selected to attain ultra-fast highly efficient separations. In order reduce complexity wine extract improve recovery efficiency, reverse-phase solid-phase extraction (SPE) procedure using as sorbent new macroporous copolymer made from balanced ratio two monomers, lipophilic divinylbenzene hydrophilic N-vinylpyrrolidone (Oasis™ HLB), performed prior UHPLC–PDA analysis. The calibration curves metabolites showed good linearity within established range. Limits (LOD) quantification (LOQ) ranged 0.006 μg mL −1 0.58 μg mL , 0.019 μg mL 1.94 μg mL acids, respectively. average recoveries ± SD three levels concentration tested ( n  =  9 ) red white wines were, respectively, 89 ± 3% 90 ± 2%. repeatability expressed relative standard deviation (RSD) below 10% all assayed. validated then applied different geographical origins (Azores, Canary Madeira Islands). most abundant component analysed (−)-epicatechin followed by (−)-catechin whereas acids were found major phenolic metabolites. completely validated, providing sensitive showing satisfactory data parameters tested. Moreover, revealed approach allowing separation investigated resolution power 5 min.