Phenolic profile of Sercial and Tinta Negra Vitis vinifera L. grape skins by HPLC–DAD–ESI-MSn: Novel phenolic compounds in Vitis vinifera L. grape

摘要: Abstract This study represents the first phytochemical research of phenolic components Sercial and Tinta Negra Vitis vinifera L. The profiles V. grape skins (white red varieties, respectively) were established using high performance liquid chromatography–diode array detection–electrospray ionisation tandem mass spectrometry (HPLC–DAD–ESI-MS n ), at different ripening stages ( veraison maturity). A total 40 compounds identified, which included 3 hydroxybenzoic acids, 8 hydroxycinnamic 4 flavanols, 5 flavanones, flavonols, stilbenes, anthocyanins. For white variety, in both stages, acids flavonols main classes, representing about 80% composition. , also predominant classes (71%), but maturity, anthocyanins represented 84% As far as we know, 10 reported for time grapes, namely protocatechuic acid-glucoside, p -hydroxybenzoyl glucoside, caftaric acid vanilloyl pentoside, -coumaric acid-erythroside, naringenin hexose derivate, eriodictyol-glucoside, taxifolin-pentoside, quercetin-glucuronide-glucoside, malylated kaempferol-glucoside, resveratrol dimer. These novel identified based on their MS fragmentation profile. data valuable information that may be useful to oenological management valorise these varieties sources bioactive compounds.