Identification of new flavonols in hybrid grapes by combined liquid chromatography–mass spectrometry approaches

摘要: Grape flavonols are involved in the phenomenon of copigmentation red wines. These compounds characterised by nutraceutical properties, have antioxidant activity and studied for chemotaxonomy grapes. In general, hybrid grapes presence polyphenols often qualitatively quantitatively different from Vitis vinifera varieties. this work, 34 grape varieties (22 12 white) produced crossing V. vinifera, riparia, labrusca, lincecumii rupestris species, were studied. Compounds combining liquid chromatography/mass spectrometry (LC/MS) methods: precursor-ion neutral-loss multiple-reaction-monitoring (MRM), high-resolution mass spectrometry. Twenty-four glycoside identified, including 4 quercetin, 5 myricetin, kaempferol, 3 isorhamnetin, 2 laricitrin, syringetin dihydroflavonol derivatives; myricetin hexoside-glucuronide, O-di-hexoside, isorhamnetin rutinoside kaempferol found first time. Statistical analysis (PCA cluster analysis) divided samples four groups on basis their flavonol profiles.