Antibodies toward high-density lipoprotein components inhibit paraoxonase activity in patients with systemic lupus erythematosus.

摘要: Patients with systemic lupus erythematosus (SLE) have an increased incidence of vascular disease, and oxidative stress is recognized as important feature in this condition, despite the underlying mechanisms not being fully understood. In these patients, interaction between lipoproteins immune system has been suggested, but most studies only looked at antibodies against oxidized low-density lipoproteins. This study was undertaken to determine presence directed high-density (HDL) identify a possible association paraoxonase (PON), antioxidant enzyme present HDL. Plasma from 55 patients SLE collected IgG aHDL antiapolipoprotein A-I (aApo A-I) were assessed by enzyme-linked immunosorbent assay. Standardization method performed control population 150 healthy subjects. levels above 5 standard deviations mean considered positive. PON activity quantification p-nitrophenol formation (micromol/mL/min). had higher titers (P < 0.0001) aApo antibodies, lower than controls. There also direct correlation (r = 0.61; P 0.0001). inversely correlated 0.0129) antibody levels. Anti-HDL high isolated subsequently incubated human These reduced up maximum 70.2% 78.4%, respectively. showed SLE. associated plasma, vitro inhibition assay confirmed activity.