Folinic acid augmentation of the effects of fluoropyrimidines on murine and human leukemic cells.

摘要: Abstract The effects of the fluoropyrimidines on leukemic cells mouse and human origin have been studied in presence folinic acid. This reduced folate enhanced cytotoxicity growth inhibitory potency 5-fluorouracil (5-FUra) 5-fluoro-2′-deoxyuridine (FUdR) against all cell lines examined. used (two T- two B-cells) were affected by these only at substantially higher concentrations than found to be L1210 cells; however, enhancement activity occurred over same range acid cells. Whereas total intracellular pool increased continuously with every increment added medium, was limited. Augmentation FUdR exceeded that studied. (as defined cloning efficiency) a greater extent inhibition so an impressive lethal synergism noted; for instance, exposure nontoxic or resulted 98 99.9% kill, respectively. In contrast previous predictions, more containing pools suboptimal folate-replete Growth rate experiments showed exposed moderate initially inhibited but recovered time, whereas both acid, initial sustained. We conclude stabilizes thymidylate synthase populations this is reflected lethality suggest doses are capable inhibiting high degree will synergistic excess folates.