Phenotypic and genotypic characterization of methicillin-resistant Staphylococcus aureus (MRSA) isolated from dogs and cats at University Veterinary Hospital, Universiti Putra Malaysia

作者: L Hassan , E Aklilu , H C Chen , Z Zunita

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摘要: Methicillin-resistant Staphylococcus aureus (MRSA) is known to cause nosocomial infections and now becoming an emerging problem in veterinary medicine. The objective of the study was determine presence MRSA 100 cats dogs sampled between November 2007 April 2008 at University Veterinary Hospital, Universiti Putra Malaysia. detected 8% pets sampled. Ten percent (5/50) 6% (3/50) isolates were from cats, respectively. All possessed mecA gene found be resistant least three antimicrobials with a minimum Oxacillin MIC 8 μg/mL. One isolate (CT04) had extremely high >256 MLST type ST59 this have been reported earlier Singapore other countries as strain animal community-associated Pulsed-field gel electrophoresis revealed five pulsotypes. Two (CT27 CT33) (DG16, DG20, DG49) respectively assigned pulsotypes B D. suggests that Malaysia are potential reservoirs for MRSA.

参考文章(33)
G. R. Ghaznavi, M. Sasikumar, S. Zamberi, S. Mariana, V. Neela, In vitro activities of 28 antimicrobial agents against methicillin-resistant Staphylococcus aureus (MRSA) from a clinical setting in Malaysia. Southeast Asian Journal of Tropical Medicine and Public Health. ,vol. 39, pp. 885- 892 ,(2008)
Hare Krishna Tiwari, Malay Ranjan Sen, Emergence of vancomycin resistant Staphylococcus aureus (VRSA) from a tertiary care hospital from northern part of India. BMC Infectious Diseases. ,vol. 6, pp. 156- 156 ,(2006) , 10.1186/1471-2334-6-156
K Murakami, W Minamide, K Wada, E Nakamura, H Teraoka, S Watanabe, Identification of methicillin-resistant strains of staphylococci by polymerase chain reaction. Journal of Clinical Microbiology. ,vol. 29, pp. 2240- 2244 ,(1991) , 10.1128/JCM.29.10.2240-2244.1991
F C Tenover, R D Arbeit, R V Goering, P A Mickelsen, B E Murray, D H Persing, B Swaminathan, Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. Journal of clinical microbiology. ,vol. 33, pp. 2233- 2239 ,(1995) , 10.1128/JCM.33.9.2233-2239.1995
J. Scott Weese, Joyce Rousseau, Josie L. Traub-Dargatz, Barbara M. Willey, Alison J. McGeer, Donald E. Low, Community-associated methicillin-resistant Staphylococcus aureus in horses and humans who work with horses. Javma-journal of The American Veterinary Medical Association. ,vol. 226, pp. 580- 583 ,(2005) , 10.2460/JAVMA.2005.226.580
Keiichi Hiramatsu, Longzhu Cui, Makoto Kuroda, Teruyo Ito, The emergence and evolution of methicillin-resistant Staphylococcus aureus Trends in Microbiology. ,vol. 9, pp. 486- 493 ,(2001) , 10.1016/S0966-842X(01)02175-8
D. Harmsen, H. Claus, W. Witte, J. Rothganger, H. Claus, D. Turnwald, U. Vogel, Typing of methicillin-resistant Staphylococcus aureus in a university hospital setting by using novel software for spa repeat determination and database management Journal of Clinical Microbiology. ,vol. 41, pp. 5442- 5448 ,(2003) , 10.1128/JCM.41.12.5442-5448.2003
So Hyun Kim, Wonki Bae, Ji Youn Lim, Ji Yeon Kim, Jun Man Kim, Soon Keun Hong, Yong Ho Park, Nam Hoon Kwon, Kun Taek Park, Woo Kyung Jung, Hwa Young Youn, Yeonhee Lee, Characteristics of methicillin resistant Staphylococcus aureus isolated from chicken meat and hospitalized dogs in Korea and their epidemiological relatedness. Veterinary Microbiology. ,vol. 117, pp. 304- 312 ,(2006) , 10.1016/J.VETMIC.2006.05.006
W. Lilenbaum, E.L.C. Nunes, M.A.I. Azeredo, Prevalence and antimicrobial susceptibility of staphylococci isolated from the skin surface of clinically normal cats Letters in Applied Microbiology. ,vol. 27, pp. 224- 228 ,(1998) , 10.1046/J.1472-765X.1998.00406.X