In vitro transfer of methicillin resistance determinants mec A from methicillin resistant Staphylococcus aureus (MRSA) to methicillin susceptible Staphylococcus aureus (MSSA)

摘要: Staphylococcus aureus more than any other human pathogen is a better model for the study of adaptive evolution bacterial resistance to antibiotics, as it has demonstrated remarkable ability in its response new antibiotics. This was designed investigate vitro transfer mecA gene from methicillin resistant S. susceptible aureus. The recipient transconjugants were erythromycin, cefpodoxime and positive. PCR amplification after mix culture plating on Luria Bertani agar containing 100 μg/mL showed that 75% donor 58.3% Additionally, 61.5% both cells positive, while 46.2% 41.75% positive LB 50 μg/mL 30 μg/mL respectively. In this study, direction phenotypic well observed have occurred strains. affirmed importance horizontal events dissemination antibiotics among different strains MRSA.