Matrix Composition and Mechanics of Decellularized Lung Scaffolds

摘要: The utility of decellularized native tissues for tissue engineering has been widely demonstrated. Here, we examine the production lung scaffolds from rodent using two different techniques, principally defined by use either detergent 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) or sodium dodecyl sulfate (SDS). All viable cellular material is removed, including at least 99% DNA. Histochemical staining and mechanical testing indicate that collagen elastin are retained in matrices with CHAPS-based decellularization, while SDS-based decellularization leads to loss decline strength. Quantitative assays confirm most CHAPS treatment but about 80% lost SDS treatment. In contrast, both methods, 60% content along 95% proteoglycan content. Mechanical indicates they mechanically similar tensile strength elastic behavior, demonstrating importance mechanics. With integrity significantly diminished some function as well. Finally, a simple theoretical model peripheral matrix mechanics consonant our experimental findings. This work demonstrates feasibility producing scaffold can be used study biology mechanics, independent effects components.