Fluorescence polarization as a means for determination of fumonisins in maize.
作者： Chris M. Maragos , Michael E. Jolley , Ronald D. Plattner , Mohammad S. Nasir
DOI: 10.1021/JF0010782
关键词:
摘要: Fumonisins, mycotoxins produced by certain species of Fusaria, are commonly found worldwide as contaminants in maize. This paper reports the development a rapid, portable fluorescence polarization-based assay for fumonisins The was based on competition unlabeled fumonisin, from sample, with fluorescently tagged fumonisin (FB1-FL) fumonisin-specific monoclonal antibody solution. polarization (FP) increased upon binding antibody. In presence free toxin, less FB1-FL bound and signal decreased. assays were very simple to perform, requiring only mixing an aqueous extract maize antibody, required <2 min per excluding extraction time. Two permutations tested, one each sample matrix serving its own blank, other all samples compared relative PBS blank normalization data s...
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