Optimization of a fluorescence polarization immunoassay for rapid quantification of deoxynivalenol in durum wheat-based products.
作者： VINCENZO LIPPOLIS , MICHELANGELO PASCALE , ANGELO VISCONTI
DOI: 10.4315/0362-028X-69.11.2712
关键词: Fluorescence polarization immunoassay 、 Botany 、 Chemistry 、 Chromatography 、 Fluorescence anisotropy
摘要: A fluorescence polarization immunoassay previously described for deoxynivalenol (DON) screening in wheat was optimized for the rapid quantification of DON in durum wheat kernels, semolina, and pasta. A background signal was observed in both spiked and naturally contaminated samples, strictly depending on the testing matrix. After subtracting the background DON level for durum wheat (0.27 μg of DON per g), semolina (0.08 μg of DON per g), and pasta (0.04 μg of DON per g), an accurate quantification of DON was …
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