Production of a monoclonal antibody with specificity for deoxynivalenol, 3‐acetyldeoxynivalenol and 15‐acetyldeoxynivalenol
作者： Maurice J. Nicol , Denis R. Lauren , Christopher O. Miles , William T. Jones
DOI: 10.1080/09540109309354799
关键词:
摘要: Monoclonal antibodies reactive with deoxynivalenol were generated following the immunization of mice a deoxynivalenol‐mouse serum albumin conjugate. One anti‐deoxynivalenol monoclonal antibodies, designated C6–1, exhibited cross‐reactivity 3‐acetyldeoxynivalenol and 15‐acetyldeoxynivalenol but not nivalenol, T‐2 tetraol or scirpentriol. An indirect competitive ELISA based on this antibody gave 50% inhibition values 0–6 μg ml‐1 for deoxynivalenol, 0–2 10 3‐acetyldeoxynivalenol.
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