Isolation of human surfactant protein A from amniotic fluid.

作者: Cheong Kb , Jemilah M , Ton Sh , Cheong Sk , Boo Ny

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摘要: Surfactant protein A (SP-A) is one of the four known surfactant-associated proteins found in human lungs. It plays a major role determining regulation surfactant uptake and resecretion. Qualitative quantitative deficiencies SP-A may contribute to neonatal respiratory distress syndrome. The measurement its level amniotic fluid or tracheal aspirate be useful assessment replacement therapy using natural synthetic surfactants. In order develop an in-house immunoassay detect SP-A, we used discontinuous sucrose density gradient isolate from fluid. Polyacrylamide gel electrophoresis was carried out on isolates with low molecular weight markers. We successfully isolated 12 31 samples were relatively pure have about 35 kD. as immunogens raise antibodies rabbits for immunoassay.

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