作者: Egner Alexander , Geisler Claudia , Rocca Francesco
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摘要: The invention relates to a method for correcting aberration-induced imaging errors in an optical system, which comprises objective lens (14) and adaptive optics (18), the light (5) specimen (20) are selected such that, as acts on (20), measuring signal (28) from is reduced, relative change depending intensity of (5). focus region system captured over first later, second period (38, 37) determine measured value. focussed by third (39) overlapping with and/or period. A numerical value determined values used metric be optimised controlling (18).