A 3D aligning method for stimulated emission depletion microscopy using fluorescence lifetime distribution.
作者： Yifan Wang , Cuifang Kuang , Shuai Li , Xiang Hao , Yingke Xu
DOI: 10.1002/JEMT.22420
关键词:
摘要: Optimal resolution by stimulated emission depletion (STED) microscopy requires precise alignment of the donut-shaped focus to excitation focus. In this article, we demonstrate that fluorescence lifetime distribution can be implemented align STED system. Different from traditional aligning methods in which a scattering imaging module is often equipped, lifetime-based method free probable mismatches between mode and fluorescent mode, drift errors caused separate complex fitting methods. Based on method, spatial 38 nm time-gated detection has been achieved. Microsc. Res. Tech. 77:935–940, 2014. © 2014 Wiley Periodicals, Inc.
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