Validation of mathematical model with phosphate activation effect by batch (R)-phenylacetylcarbinol biotransformation process utilizing Candida tropicalis pyruvate decarboxylase in phosphate buffer.

摘要: The (R)-phenylacetylcarbinol (PAC) batch biotransformation kinetics for partially purified Candida tropicalis TISTR 5350 pyruvate decarboxylase (PDC) were determined to validate a comprehensive mathematical model in 250 mL scale with 250 mM phosphate buffer/pH 7.0. PDC could convert initial 100/120 mM benzaldehyde/pyruvate substrates the statistical significantly highest (p ≤ 0.05) maximum PAC concentration (95.8 ± 0.1 mM) and production rate (0.639 ± 0.001 mM min-1). A parameter search strategy aimed at minimizing overall residual sum of square (RSST) based on system six ordinary differential equations was applied profiles 100/120 30/36 mM. Ten important kinetic parameters then elucidated including zeroth order activation constant due buffer species (ka) (9.38 ±  < 0.01) ×  10-6% relative activity min-1 mM-1. validation this independent 50/60 mM resulted relatively good fitting RSST, mean error (MSE), coefficient determination (R2) values 662, 17.4, 0.9863, respectively.