Detection of carboxymethylmetallothionein by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
作者： Masami Kimura , Shinzi Koizumi , Fuminori Otsuka
DOI: 10.1016/0076-6879(91)05093-B
关键词:
摘要: A sensitive method for detecting metallothioneins (MTs) by using silver staining and autoradiography after sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of carboxymethylated MTs is described. Carboxymethylation indispensable because it prevents their aggregation, thereby allowing each them to appear as a single band SDS-PAGE. Metallothioneins can be detected with limit nanogram levels per lane. This applied induced in vitro cultured cells vivo tissues.
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