A new biomarker for mitotic cells

摘要: Many epitopes are phosphorylated during mitosis. These useful biomarkers for mitotic cells. The most commonly used MPM-2 and serine 10 of histone H3. Here we investigated the use an antibody generated against a phospho peptide matching residues 774–788 human retinoblastoma protein 1 (Rb) to detect Human cell lines were stained with DNA dyes antibodies reactive defined by MPM-2, phospho-S10-histone-H3, phospho-serine peptide, TRPPTLSPIPHIPRC (phospho-S780-Rb). Immunoreactivity content measured flow image cytometry. Correlation pattern recognition analyses performed on list mode data. Western blots immunoprecipitation investigate number peptides phospho-S780-Rb relationship between reactivity this MPM-2. Costaining bromodeoxyuridine (BrdU) was determine acid resistance epitope. Cell cycle related immunofluorescence correlated strongly that Laser scanning cytometry showed is expressed at high levels all stages blotting epitope several including Rb protein. BrdU stable acid. Kinetic experiments utility in complex analysis aimed measuring transition state timing. robust marker mitosis allows cytometric detection cells beginning chromatin condensation ending after cytokinesis. discrimination counting post-cytokinetic (‘‘newborn’’) To facilitate without confusion about specificity, suggest trivial name, pS780 ' 2007 International Society Analytical Cytology