Pooled CRISPRi screening of the cyanobacterium Synechocystis sp PCC 6803 for enhanced industrial phenotypes.

作者: Lun Yao , Kiyan Shabestary , Sara M. Björk , Johannes Asplund-Samuelsson , Haakan N. Joensson

DOI: 10.1038/S41467-020-15491-7

关键词:

摘要: … clones for the model cyanobacterium Synechocystis sp. PCC … into how to engineer cyanobacteria metabolism for industrial … can improve bioproduction in cyanobacteria. By screening …

参考文章(69)
Michael I Love, Wolfgang Huber, Simon Anders, Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2 Genome Biology. ,vol. 15, pp. 550- 550 ,(2014) , 10.1186/S13059-014-0550-8
Wei Xiong, Tai-Chi Lee, Sarah Rommelfanger, Erica Gjersing, Melissa Cano, Pin-Ching Maness, Maria Ghirardi, Jianping Yu, Phosphoketolase pathway contributes to carbon metabolism in cyanobacteria Nature plants. ,vol. 2, pp. 15187- ,(2016) , 10.1038/NPLANTS.2015.187
Lun Yao, Ivana Cengic, Josefine Anfelt, Elton P. Hudson, Multiple Gene Repression in Cyanobacteria Using CRISPRi. ACS Synthetic Biology. ,vol. 5, pp. 207- 212 ,(2016) , 10.1021/ACSSYNBIO.5B00264
Isabel Orf, Stefan Timm, Hermann Bauwe, Alisdair R. Fernie, Martin Hagemann, Joachim Kopka, Zoran Nikoloski, Can cyanobacteria serve as a model of plant photorespiration? – a comparative meta-analysis of metabolite profiles Journal of Experimental Botany. ,vol. 67, pp. 2941- 2952 ,(2016) , 10.1093/JXB/ERW068
Xi Chen, Karoline Schreiber, Jens Appel, Alexander Makowka, Berit Fähnrich, Mayo Roettger, Mohammad R. Hajirezaei, Frank D. Sönnichsen, Peter Schönheit, William F. Martin, Kirstin Gutekunst, The Entner–Doudoroff pathway is an overlooked glycolytic route in cyanobacteria and plants Proceedings of the National Academy of Sciences of the United States of America. ,vol. 113, pp. 5441- 5446 ,(2016) , 10.1073/PNAS.1521916113
Jason M. Peters, Alexandre Colavin, Handuo Shi, Tomasz L. Czarny, Matthew H. Larson, Spencer Wong, John S. Hawkins, Candy H.S. Lu, Byoung-Mo Koo, Elizabeth Marta, Anthony L. Shiver, Evan H. Whitehead, Jonathan S. Weissman, Eric D. Brown, Lei S. Qi, Kerwyn Casey Huang, Carol A. Gross, A Comprehensive, CRISPR-based Functional Analysis of Essential Genes in Bacteria Cell. ,vol. 165, pp. 1493- 1506 ,(2016) , 10.1016/J.CELL.2016.05.003
Kiyan Shabestary, Elton P. Hudson, Computational metabolic engineering strategies for growth-coupled biofuel production by Synechocystis. Metabolic Engineering Communications. ,vol. 3, pp. 216- 226 ,(2016) , 10.1016/J.METENO.2016.07.003
Alice J. de Porcellinis, Stephan Klähn, Lisa Rosgaard, Rebekka Kirsch, Kirstin Gutekunst, Jens Georg, Wolfgang R. Hess, Yumiko Sakuragi, The Non-Coding RNA Ncr0700/PmgR1 is Required for Photomixotrophic Growth and the Regulation of Glycogen Accumulation in the Cyanobacterium Synechocystis sp. PCC 6803 Plant and Cell Physiology. ,vol. 57, pp. 2091- 2103 ,(2016) , 10.1093/PCP/PCW128
Gina C. Gordon, Travis C. Korosh, Jeffrey C. Cameron, Andrew L. Markley, Matthew B. Begemann, Brian F. Pfleger, CRISPR interference as a titratable, trans-acting regulatory tool for metabolic engineering in the cyanobacterium Synechococcus sp. strain PCC 7002 Metabolic Engineering. ,vol. 38, pp. 170- 179 ,(2016) , 10.1016/J.YMBEN.2016.07.007
Neal J Oliver, Christine A Rabinovitch-Deere, Austin L Carroll, Nicole E Nozzi, Anna E Case, Shota Atsumi, Cyanobacterial metabolic engineering for biofuel and chemical production. Current Opinion in Chemical Biology. ,vol. 35, pp. 43- 50 ,(2016) , 10.1016/J.CBPA.2016.08.023