Effects of different mixtures of fibrolytic enzymes on digestion and fermentation of bahiagrass hay

摘要: Abstract This study was designed to determine the best combination of ferulic acid esterase (FAE), cellulose (CEL), and xylanase (XYL) for hydrolyzing mature bahiagrass in absence rumen fluid, evaluate effects multienzyme cocktails on digestibility fermentation presence fluid. In Experiment 1, 42 enzyme mixtures pure CEL, XYL, FAE were dissolved a citrate phosphate buffer (pH 6.0) added 0.5 g 12-week regrowth ( Paspalum notatum ) hay. each mixture, applied at 0, 0.5, or 2 g/100 g dry matter (DM) except that not DM. Dry disappearance measured after solutions incubated 39 °C 24 h. Each triplicate experiment repeated thrice. The highest DM with containing 2, 0 (2–2–0) 1 (2–2–1) g FAE, respectively/100 g forage hay treated nothing (Control), commercial preparation (Depol 740, D740L) all three enzymes, 2–2–0 2–2–1 from 1. 1 ml citrate–phosphate sprayed two runs. Samples fermented buffered-rumen fluid using wireless automated gas production system either 24 96 h duplicate runs, residues analyzed volatile fatty concentrations. kinetics determined by fitting non-linear model data. For 24 h fermentations, application did increase NDF but decreased acetate concentration (P