Gene expression and inducibility of the aryl hydrocarbon receptor-dependent pathway in cultured bovine blood lymphocytes.

摘要: Abstract The exposure to dioxin-like (DL) compounds, an important class of persistent environmental pollutants, results in the altered expression target genes. This occurs through binding aryl hydrocarbon receptor (AhR), subsequent dimerization with AhR nuclear translocator (ARNT), and complex DNA responsive elements. A number genes are up-regulated, including, among others, repressor (AHRR) several biotransformation enzymes, such as members CYP1 family NAD(P)H-quinone oxidoreductase (NOQ1). inducibility above were investigated mitogen-stimulated cultured blood lymphocytes from cattle, which represent a notable source DL-compound human dairy products meat. As assessed by real-time PCR, all examined except CYP1A2 NQO1 detected under basal conditions. Cell DL-compounds PCB126 or PCB77 10−6–10−9 M concentration range resulted 2–4-fold induction CYPIA1 CYP1B1, was antagonized α-naphthoflavone PCB153. study demonstrates for first time presence pathway easily accessible cells like bovine peripheral prompts further investigations verify whether similar changes could occur vivo