Identification of candidate biomarkers of the exposure to PCBs in contaminated cattle: A gene expression- and proteomic-based approach.

摘要: Dioxins and polychlorinated biphenyls (PCBs) are widespread persistent contaminants. Through a combined gene expression/proteomic-based approach, candidate biomarkers of the exposure to such environmental pollutants in cattle subjected real eco-contamination event were identified. Animals removed from polluted area fed standard ration for 6 months. The decontamination was monitored by evaluating dioxin PCB levels pericaudal fat two weeks after removal contaminated (day 0) then bimonthly six months (days 59, 125 188). Gene expression measurements demonstrated that CYP1B1 significantly higher blood lymphocytes collected animals 0), decreased over time during decontamination. mRNA interleukin 2 showed an opposite quantitative trend. MALDI-TOF-MS polypeptide profiling serum samples ascertained progressive decrease (from day 0 188) fibrinogen β-chain serpin A3-7-like fragments, apolipoprotein (APO) C-II amyloid A-4 protein, along with augmented representation transthyretin isoforms, as well APOC-III APOA-II proteins When differentially represented species antioxidant, acute phase proinflammatory protein already same (Cigliano et al., 2016), bioinformatics unveiled interaction network linking together almost all components. This suggests occurrence complex PCB-responsive mechanism associated animal contamination/decontamination, including cohort protein/polypeptide involved redox homeostasis, inflammation lipid transport. All together, these results suggest use combination identifying PCB-contaminated animals, monitoring restoring their healthy condition following process.