Multidrug resistance-associated protein 7 expression is involved in cross-resistance to docetaxel in salivary gland adenocarcinoma cell lines

摘要: The aim of the present study was to clarify whether ATP binding cassette transporters are refractory factors in head and neck cancers. For vitro vivo chemotherapeutic studies, we used following cancer cell lines: a mouse oral squamous carcinoma (SCC) line, Sq-1979; human SCC SCCHA; salivary gland adenocarcinoma (SGA) NR-PG; SGA HSY. We vinca alkaloid anticancer drug, vincristine (VCR), as drug. To determine cause multidrug resistance, Western blot analysis, reverse transcription-polymerase chain reaction (RT-PCR), immunohistochemistry xenografted tumors nude mice, drug efflux inhibitory assays were performed. VCR-treated lines, Sq-1979/VCR, SCCHA/VCR, NR-PG/VCR, HSY/VCR, intensively expressed resistance (MDR) gene 1 mRNA associated protein (MRP) mRNA. MRP7 NR-PG/VCR HSY/VCR cells, but not Sq-1979/VCR SCCHA/VCR cells. In each clone induced by VCR treatment, suggesting an acquired context expression. mice model, SCCHA HSY cells MDR1 MRP1. Moreover, expression immunohistochemically found VCR-injected tumor-bearing Furthermore, doxorubicin accumulation increased cross-resistance docetaxel decreased presence competitive inhibitor, 17-beta-estradiol-(17-beta-D-glucuronide). These results indicate that expression, MRP1 chemotherapy suggest induction is involved natural products, especially SGA.