Simultaneous estimation of Cefixime and Ofloxacin in tablet dosage form by RP- HPLC

摘要: A simple, precise, accurate RP-HPLC method has been developed for the simultaneous estimation of cefixime and ofloxacin in combined tablet dosage form. The chromatographic separation was achieved using Phenomenex Luna C18 (250 X 4.60 mm, 5 µm) analytical column mobile phase consisting methanol 25 mM phosphate buffer 40: 60% (v/v) pH adjusted to 5.5 ortho phosphoric acid at a flow rate 1.2 mL/min. UV detection carried out 290 nm photodiode array detector. retention time found be 2.5 min 7.8 respectively. proposed validated specificity, linearity, accuracy, precision, ruggedness, robustness, limit quantitation. Linear calibration curves were obtained concentration 5-25 µg/mL ofloxacin. mean percentage recovery respectively between 98.0% 105.7%. Limit quantification 13.3 ng/mL 40.1 12.3 37.1 ng/mL, can used routine analysis titled drugs combination formulation.