Gene therapy for human small-cell lung carcinoma by inactivation of Skp-2 with virally mediated RNA interference

摘要: Increase of Skp-2, which is involved in the degradation cell cycle regulators including p27Kip1, p21 and c-myc, one important mechanisms for dysregulation cycles various cancers. We applied RNA interference (RNAi) Skp-2 by using HIV-lentiviral or adenoviral vectors a human small-cell lung carcinoma line with increased to evaluate RNAi strategy cancer gene therapy. HIV-lentivirus-mediated resulted efficient inhibition vitro growth cells through increase p27Kip1 p21, but no significant effect on without high expression. Furthermore, intratumoral administration adenovirus siRNA vector efficiently inhibited established subcutaneous tumor NOD/SCID mice. These results indicate that may be useful therapy cancers