Crystallization experiments with 2-enoyl-CoA hydratase, using an automated 'fast-screening' crystallization protocol.

摘要: A convenient method for screening crystallization conditions using an automated fast-screen protocol has been implemented and tested on enoyl-CoA hydratase. The solutions the initial subsequent optimizations are prepared a robot. Enoyl-CoA hydratase (E.C. 4.2.1.17), purified from rat-liver mitochondria, is one of enzymes β-oxidation pathway fatty-acid metabolism; it catalyzes reversible hydration 2-trans-enoyl-CoA's to l-3-hydroxy-acyl-CoA's. Different crystal forms, diffracting 3.0 A, were obtained.