Discovery of Lipidome Alterations Following Traumatic Brain Injury via High-Resolution Metabolomics.

摘要: Traumatic brain injury (TBI) can occur across wide segments of the population, presenting in a heterogeneous manner that makes diagnosis inconsistent and management challenging. Biomarkers offer potential to objectively identify status, severity, phenotype by measuring relative concentrations endogenous molecules readily accessible biofluids. Through data-driven, discovery approach, novel biomarker candidates for TBI were identified serum lipidome adult male Sprague–Dawley rats first week following moderate controlled cortical impact (CCI). Serum samples analyzed positive negative modes ultraperformance liquid chromatography–mass spectrometry (UPLC–MS). A predictive panel classification injured uninjured sera samples, consisting 26 dysregulated species belonging variety lipid classes, was developed with cross-validated accuracy 85.3% using omniClassifier software optimize feature selection. Polyunsaturated fatty a...