The mRNA export factor Dbp5 is associated with Balbiani ring mRNP from gene to cytoplasm

摘要: The DEAD box RNA helicase Dbp5 is essential for nucleocytoplasmic transport of mRNA–protein (mRNP) complexes. present mainly in the cytoplasm and enriched at cytoplasmic side nuclear pore complexes (NPCs), suggesting that it acts late part mRNP export. Here, we visualize assembly a specific particle, Balbiani ring dipteran Chironomus tentans, show homologue C.tentans, Ct-Dbp5, binds to pre-mRNP co-transcriptionally accompanies through pores into cytoplasm. We also demonstrate Ct-Dbp5 accumulates nucleus partly disappears from NPC when export mRNA inhibited. fact along exiting fibril extending supports view involved restructuring prior translation. Finally, addition factor growing transcript highlights importance co-transcriptional loading process determining fate mRNA.