Gene Expression in Polytene Nuclei

摘要: Gene expression in eukaryotic cells is a multi-step process. Many of the steps are both co-ordinated and quality controlled. For example, transcription closely coupled to pre-messenger RNA (mRNA)-protein assembly, pre-mRNA processing, surveillance correct synthesis messenger ribonucleoprotein (mRNP), export. The coordination appears be exerted through dynamic interactions between components transcription, surveillance, export machineries. Our knowledge so far incomplete about these molecular where nucleus they take place. It therefore essential analyze intranuclear gene vivo. Polytene nuclei exceptionally large contain chromosomes individual genes that can structurally analyzed situ during ongoing transcription. Furthermore, gene-specific pre-mRNPs/mRNPs visualised as synthesised on then followed their path cytoplasm. We describe methods for investigating structure composition active chromatin context analyses processes polytene cells.