Clonogenic cell survival in cryopreserved human tumour cells.

摘要: Cells from 3 human tumours have been grown in soft agar contained Millipore diffusion chambers and implanted i.p. mice. Clonal growth was obtained fresh biopsy samples, cryopreserved tissue, xenografts of the tissues immune-suppressed The radiosensitivities a melanoma an ovarian carcinoma were evaluated by vitro irradiation before assay for colony formation. Xenografting did not modify radiosensitivity melanoma. another tumour exposed to Adriamycin or cyclophosphamide whilst within chambers, sensitivity similar xenografted cells. results encourage further attempts quantify cells these methods.