Identification and characterization of novel rat and human gonad-specific organic anion transporters.

摘要: We have isolated three novel organic anion transporter cDNAs designated rat GST-1 (gonad-specific transporter), GST-2, and human GST, expressed at high levels in the testis. Rat GST-1, GST consist of 748, 702, 719 amino acids, respectively, all molecules possess 12 predicted transmembrane domains, which is a common structure transporters. Northern blot analyses situ hybridization revealed that both are highly testis, especially Sertoli cells, spermatogonia, Leydig cells. Weak signals also detected epididymis ovary adult rat. The exclusive expression mRNA testis was confirmed by RT-PCR. pharmacological experiments Xenopus laevis oocytes injected with respective GST-1- GST-2-cRNAs GST-2 transport taurocholic acid, dehydroepiandrosterone sulfate, T4 Michaelis-Menten kinetics (taurocholic Km = 8.9 2.5 ...