Characterization of a mouse model of headache.

摘要: Migraine and other primary headache disorders affect a large population cause debilitating pain. Establishing animal models that display behavioral correlates of long-lasting ongoing headache, the most common disabling symptom migraine, is vital for elucidation disease mechanisms identification drug targets. We have developed mouse model using dural application capsaicin along with mixture inflammatory mediators (IScap) to simulate induction episode. This elicited intermittent head-directed wiping scratching as well phosphorylation c-Jun N-terminal kinase in trigeminal ganglion neurons. Interestingly, IScap preferentially induced FOS protein expression excitatory but not inhibitory cervical/medullary dorsal horn The duration IScap-induced behavior number FOS-positive neurons correlated positively individual mice; both were reduced control level by pretreatment antimigraine sumatriptan. Dural CGRP(8-37), calcitonin gene-related peptide (CGRP) receptor antagonist, also effectively blocked behavior, which suggests release endogenous CGRP dura necessary nociception. These data suggest nocifensive mice may be mechanistically related humans. In addition, increased resting time female mice. Taken together, we present first detailed study can applied genetically modified facilitate research on therapeutic targets migraine headache.