Effect of Menopause on Gene Expression Profiles of Circulating Monocytes: A Pilot in vivo Microarray Study

摘要: Menopause is one of the key physiological events in female life and can increase risk for a number complex autoimmune, neurodegenerative, metabolic, cardiovascular disorders. Circulating monocytes differentiate into various cell types play an important role tissue morphogenesis immune response. We studied gene expression profiles peripheral blood healthy pre- postmenopausal women using Affymetrix Human U133A GeneChip array that contains probes approximately 14,500 genes. Comparative analyses between samples showed 20 genes were up- down-regulated. Of these genes, 28 classified six major GO categories relevant to such biological processes as proliferation, response, cellular metabolism, others. The remaining 12 have yet unidentified functions. Our results support hypothesis functional state circulating indeed affected by menopause, resulting changes may be determined through genomewide profiling. Several differentially expressed identified this study candidates further studies menopause-associated systemic only first attempt direction, but it lays basis research.