Solvatochromic parameter values and pH in acetonitrile-water mixtures optimization of mobile phase for the separation of peptides by high-performance liquid chromatography☆
作者： J. Barbosa , V. Sanz-Nebot , I. Toro
DOI: 10.1016/0021-9673(95)00851-9
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摘要: Abstract The proportion of the organic modifier and pH mobile phase were optimized in order to separate a series low-molecular-mass peptides by HPLC. composition was establishing relationships between retention parameters Reichardt's E T N scale solvent polarity, Kamlet-Taft multiparameter eluent, using linear solvation energy (LSER). also successfully measured aqueous-organic mixture used as eluent.
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