Measurement of glycosylated hemoglobins using boronate affinity chromatography

摘要: A boronate affinity column method for the measurement of glycosylated hemoglobins was evaluated. In procedure were bound by immobilized boronic acid to separate them from nonglycosylated hemoglobins. Elution carried out with sorbitol buffer, and absorbance read at 414 nm. The linear a hemoglobin concentration least 20 percent. precision ranged 1.2 2.8 percent (C.V.) within-run, 3.4 5.3 day-to-day. reference interval 4.8 6.4 correlated cation exchange resin mini-column (r = 0.94) colorimetric 0.93) but results higher in diabetic patients. measured significantly estimated one-day-mean plasma glucose patients 0.54, n 52, p less than 0.002). provides an attractive alternative earlier methods measuring