DNA-binding specificity of the fushi tarazu homeodomain.
作者： B Florence , R Handrow , A Laughon
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摘要: The fushi tarazu (ftz) gene of Drosophila melanogaster encodes a homeodomain-containing transcription factor that functions in the formation body segments. Here we report an analysis DNA-binding properties ftz homeodomain vitro. We provide evidence binds to DNA as monomer, with equilibrium dissociation constant 2.5 x 10(-11) M for binding consensus site. A single site occupies 10 12 bp, judged by ability protein bound at one interfere adjacent These experiments also demonstrated lack cooperative between homeodomains. Analysis single-nucleotide substitutions over 11-bp sequence shows stretch 6 bp is critical binding, optimal 5'CTAATTA3'. data correlate well recent structural base-specific contact these positions. In addition, found sequences flanking region direct have effects on could be biological significance.
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