High-performance liquid chromatography with fluorescence detection for the simultaneous determination of 3,4-methylenedioxymethamphetamine, methamphetamine and their metabolites in human hair using DIB-Cl as a label

摘要: This paper describes a highly sensitive HPLC method for the simultaneous determination of 3,4-methylenedioxymethamphetamine (MDMA), 3,4-methylenedioxyamphetamine (MDA), amphetamine (AP) and methamphetamine (MP) in human hair samples. The amphetamines investigated were derivatized with fluorescent reagent, DIB-Cl to yield DIB-derivatives, which then analyzed by fluorescence detection at excitation emission wavelengths 325 nm 430 nm, respectively. separation was achieved on an ODS column isocratic mobile phase composed acetonitrile-methanol-water (30:40:30, v/v/v). limits four compounds obtained proposed ranged from 11 200 pg/mg. successfully applied MDMA MDA samples abuser.