Detection of early developmental stages of Myxobolus cerebralis in fish and tubificid oligochaete hosts by in situ hybridization.

摘要: The myxosporean and actinosporean spores of Myxobolus cerebralis develop through many stages in their respective hosts, salmonid fishes a tubificid oligochaete. Using modified, non-radioactive situ hybridization protocol, the parasite, which exhibits radically different structural forms during its development each host, could be specifically detected paraffin-embedded tissues both fish oligochaetes. Our study aims to demonstrate application technique for detection early M. hosts.