Experimentally induced whirling disease. II: Determination of longevity of the infective triactinomyxon stage of Myxobolus cerebralis by vital staining

摘要: Abstract Effects of temperature and aging on viability infectivity laboratory-produced actinosporean triactinomyxon spores (infective stage the organism causing whirling disease) were studied. In vitro staining with vital fluorescein diacetate correlated ability to infect fry rainbow trout Oncorhynchus mykiss. The experimentally produced Myxobolus cerebralis was short-lived, persisting for only 3–4 d at 12.5°C less time warmer temperatures. method has potential screening therapeutants intended control myxosporean infection fish.