Binding of Glycosaminoglycans to Leukocytes Using Fluorescent Labeled Gag-Derivatives

摘要: The non-anticoagulant properties of glycosaminoglycans (GAGs) include antithrombotic, antiatherosclerotic, antiinflammatory and antimetastatic actions are thought to be caused by binding the GAGs positively charged groups matrices peptides (1, 2, 3). Heparins best known group they linear polysaccharide chains with defined negatively sulfate carboxylic groups. Fractions fragments heparins heparin-derived oligosaccharides were obtained investigate metabolism structure function relationship plasma cellular proteins. Binding heparin human endothelial cells was demonstrated using radiolabeled compounds (4). Decreasing molecular weight chains, decreased (5). Heparin has been reported bind monocytes modulate their procoagulant activity Blood macrophages found internalize degrade (6). Unfractionated seems erythrocytes as radioactively labeled compound (7).