Preparation and properties of fluorescent polysaccharides.

摘要: A new method for preparing fluorescein derivatives of polysaccharides is described. These are prepared by activation the polysaccharide with cyanogen bromide and subsequent reaction fluoresceinamine. The optimum conditions coupling have been established in this report. Using procedure, we a wide variety polysaccharides. Degrees substitution range 3.0 X 10(-3) to 2.4 10(-2) mol per mole monosaccharide equivalent were obtained. fluorescent stable: no free was detected after incubation at 22 degrees C 48 h or -10 4 months. fluorescein-derivatized found same potency inhibiting lectin-mediated hemagglutination as underivatized polysaccharide. In addition, these can be radioiodinated specific activities exceeding 10(6) dpm/micrograms due incorporation 125I into fluorescein. cell binding properties 125I-fucoidin 125I-heparin indistinguishable from corresponding This general approach should produce useful reagents localizing quantifying surface carbohydrate-binding proteins (lectins).