Pro and antioxidant responses to repeated administration of diazepam in rat brain.
作者： Sarah Musavi , Poonam Kakkar
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摘要: The role of pro/antioxidative processes during a low, subtoxic dose schedule diazepam (3 mg/kg/day i.p.) for 7 days and its withdrawal in subcellular preparations rat brain regions was studied detail. results indicated heterogeneity the regional responses as well compartments. After exposure to drug, decrease Mn-SOD activity observed 3 while significant increase Cu/Zn-SOD seen cerebellum (CBL) stem (BS) along with that mitochondrial glutathione reductase. post-mitochondrial fraction (PMF) showed GR cerebrum. Enhancement total free thiol levels cerebrum whereas BS thiols were not enhanced. It interesting note animals withdrawn from drug sacrificed after an interval days, level TBARS highly mitochondria CB CBL 89% BS. Similar trend fractions all isozymes SOD decreased (p < 0.001) lesser extent CB. significantly only 34% rise no change PMFs but 20% Thus, data show modulation antioxidant short-term administration diazepam, lowering peroxidative decomposition polyunsaturated fatty acids membranes. However, PUFAs found be more vulnerable changes defenses also observed, which did come back normal study.
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