X-chromosome inactivation and epigenetic fluidity in human embryonic stem cells

摘要: With the potential to give rise all somatic cell types, human embryonic stem cells (hESC) have generated enormous interest as agents of replacement therapy. One limitation is their safety in vivo. Although several studies focused on concerns over genomic stability ex vivo, few analyzed epigenetic stability. Here, we use tools phenomenon, X-chromosome inactivation (XCI), investigate properties. Among 11 distinct hESC lines, find a high degree variability. We show that, like mouse ESC, principle capacity recapitulate XCI when induced differentiate culture (class I lines). However, this seen isolates. Many lines already undergone II and III). Unexpectedly, there tendency lose XIST RNA expression during Despite losing H3-K27 trimethylation, inactive X class III remains transcriptionally suppressed, indicated by Cot-1 exclusion. conclude that are subject dynamic reprogramming Given differentiation tightly linked, consider implications for pluripotency potential.