A monoclonal antibody (HML-1) defining a novel membrane molecule present on human intestinal lymphocytes

摘要: A monoclonal antibody, HML-1, was produced by fusion of NSI myeloma cells with spleen a mouse immunized isolated human intestinal intraepithelial lymphocytes (IEL). Immunofluorescence studies cells, as well immunoperoxidase staining tissue sections, indicated that HML-1 labeled all the various subsets IEL, approximately 40% lamina propria T 30% mesenteric lymphoblasts and some in other mucosae, particularly IEL. Conversely, it revealed only rare lymphoid compartments. Analysis polyacrylamide gel gradient electrophoresis showed precipitated two major noncovalently bound components approximate mol. masses 105 150 kDa from thus defines novel membrane antigen present on subpopulation preferentially associated epithelia, epithelium. The characteristics this are very similar to those an we had previously described rat. possible functional role class lymphocyte antigens nature mechanism triggers their expression remain be elucidated.