A new selective medium for the culture of clostridia from human faeces.

作者: N. de Vos , E. Mevissen-Verhage , W. Harmsen van Amerongen , J. Marcelis

DOI: 10.1007/BF02019969

关键词:

摘要: A new selective medium, sulphite-polymixin-milk (SPM) agar, for the isolation of clostridia from faeces is described. This medium contains whole cow's milk, which favours growth over that ofBacteroides andBifidobacterium spp., and only small amounts colistin (10μg/ml) suppressing coliforms. The alkaline end-products give rise to yellow colonies on a red background are easy distinguish other not completely inhibited bacteria. suitable many species. Comparisons were made between SPM several media recovery clostridia. detection limit agar was more than 103 below Reinforced Clostridia Agar (Oxoid CM101).

参考文章(18)
Robert Angelotti, Herbert E. Hall, Milton J. Foter, Keith H. Lewis, Quantitation of Clostridium perfringens in foods. Applied and Environmental Microbiology. ,vol. 10, pp. 193- 199 ,(1962) , 10.1128/AM.10.3.193-199.1962
Robert S. Marshall, J. Frank Steenbergen, L. S. McClung, Rapid Technique for the Enumeration of Clostridium perfringens Applied Microbiology. ,vol. 13, pp. 559- 563 ,(1965) , 10.1128/AM.13.4.559-563.1965
Lillian V. Holdeman, Elizabeth P. Cato, Walter Edward C. Moore, Anaerobe Laboratory manual Anaerobe Laboratory, Virginia Polytechnic Institute and State University. ,(1977)
M Dezfulian, L M McCroskey, C L Hatheway, V R Dowell, Selective medium for isolation of Clostridium botulinum from human feces. Journal of Clinical Microbiology. ,vol. 13, pp. 526- 531 ,(1981) , 10.1128/JCM.13.3.526-531.1981
S. M. Finegold, Taxonomy, Enzymes, and Clinical Relevance of Anaerobic Bacteria Clinical Infectious Diseases. ,vol. 1, pp. 248- 253 ,(1979) , 10.1093/CLINIDS/1.2.248
J. G. E. M. Lindner, J. H. Marcelis, Quantitative gas chromatography of Bacteroides species under different growth conditions. Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology. ,vol. 44, pp. 1- 14 ,(1978) , 10.1007/BF00400072
D Vargo, M Moskovitz, M H Floch, Faecal bacterial flora in cancer of the colon. Gut. ,vol. 21, pp. 701- 705 ,(1980) , 10.1136/GUT.21.8.701
A. A. Miles, S. S. Misra, J. O. Irwin, The estimation of the bactericidal power of the blood. Journal of Hygiene. ,vol. 38, pp. 732- 749 ,(1938) , 10.1017/S002217240001158X
B Frank Polk, DENNIS L KASPER, Bacteroides fragilis Subspecies in Clinical Isolates Annals of Internal Medicine. ,vol. 86, pp. 569- 571 ,(1977) , 10.7326/0003-4819-86-5-569