Evaluation of the cytotoxicity of elastomeric ligatures after sterilisation with 0.25% peracetic acid.

作者: Pithon Mm , dos Santos Rl , de Assuncao Ps , Restle L , Judice Rl

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摘要: Introduction: Sterilisation using peracetic acid (PAA) has been advocated for orthodontic elastic bands. However, cane-loaded elastomeric ligatures can also become contaminated during processing, packaging, and manipulation before placement in the oral cavity are therefore susceptible, possible causes, of cross-contamination. Aim: To test hypothesis that 0.25% (PAA), following sterilisation elastomers, influences cytotoxicity on L929 cell lines. Materials methods: Four hundred eighty silver were divided into 4 groups 120 to produce, Group TP (latex natural, bulk pack, Orthodontics), M1 (Polyurethane, Morelli), M2 cane-loaded, Morelli) U Uniden). Of each group, 100 sterilised PAA at time intervals (N = 20) 1 hour, 2 hours, 3 hours 5 hours. The 20 remaining group not served as controls. Cytotoxicity was assessed lines a dye-uptake method. Analysis variance (ANOVA), followed by Tukey post hoc (p < 0.05) determined statistical relevance. Results: There significant difference between TP, Morelli Uniden elastomerics 0.05), but no two types hour interval. In addition, there CC other assessed, except Groups non-sterilised showed similar viability observed after standard sterilisation. Conclusion: did significantly influence is recommended clinical use. (Aust Orthod J 2013; 29: 139-144)

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