Characterization and subcellular localization of the dystrophin-protein 71 (Dp71) from brain
作者： Daniel Jung , Dominique Filliol , Marie-Helene Metz-Boutigue , Alvaro Rendon
DOI: 10.1016/0960-8966(93)90107-U
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摘要: In the present study, monoclonal antibodies raised against C-terminal domain of dystrophin were used to identify and characterize Dp71 from central nervous system. It was observed that expression gradually increases embryo stage until adult. Subcellular distribution analysis indicates is mainly recovered in synaptic plasma membranes, microsomes at a lesser extent vesicles mitochondria. The amino acid composition N-terminal sequence bovine brain determined. Moreover, we found this protein glycosylated.
sci-hub.se 参考文章(14)
PH O'Farrell, High resolution two-dimensional electrophoresis of proteins. Journal of Biological Chemistry. ,vol. 250, pp. 4007- 4021 ,(1975) , 10.1016/S0021-9258(19)41496-8
James M. Ervasti, Kevin P. Campbell, Membrane organization of the dystrophin-glycoprotein complex Cell. ,vol. 66, pp. 1121- 1131 ,(1991) , 10.1016/0092-8674(91)90035-W
Alvaro Rendon, Ahmed Masmoudi, Purification of non-synaptic and synaptic mitochondria and plasma membranes from rat brain by a rapid Percoll gradient procedure Journal of Neuroscience Methods. ,vol. 14, pp. 41- 51 ,(1985) , 10.1016/0165-0270(85)90113-X
Eric Fabbrizio, Jocelyne Léger, Monique Anoal, Jean J. Léger, Dominique Mornet, Monoclonal antibodies targeted against the C-terminal domain of dystrophin or utrophin FEBS Letters. ,vol. 322, pp. 10- 14 ,(1993) , 10.1016/0014-5793(93)81100-E
D. Lederfein, Z. Levy, N. Augier, D. Mornet, G. Morris, O. Fuchs, D. Yaffe, U. Nudel, A 71-kilodalton protein is a major product of the Duchenne muscular dystrophy gene in brain and other nonmuscle tissues. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 89, pp. 5346- 5350 ,(1992) , 10.1073/PNAS.89.12.5346
Eric P. Hoffman, Robert H. Brown, Louis M. Kunkel, Dystrophin: The protein product of the duchenne muscular dystrophy locus Cell. ,vol. 51, pp. 919- 928 ,(1987) , 10.1016/0092-8674(87)90579-4
Derek J. Blake, Donald R. Love, Jonathon Tinsley, Glenn E. Morris, Helen Turley, Kevin Gatter, George Dickson, Yvonne H. Edwards, Kay E. Davies, Characterization of a 4.8kb transcript from the Duchenne muscular dystrophy locus expressed in Schwannoma cells Human Molecular Genetics. ,vol. 1, pp. 103- 109 ,(1992) , 10.1093/HMG/1.2.103
W B Huttner, W Schiebler, P Greengard, P De Camilli, Synapsin I (protein I), a nerve terminal-specific phosphoprotein. III. Its association with synaptic vesicles studied in a highly purified synaptic vesicle preparation. Journal of Cell Biology. ,vol. 96, pp. 1374- 1388 ,(1983) , 10.1083/JCB.96.5.1374
Uri Nudel, Dorit Zuk, Paz Einat, Elisha Zeelon, Zehava Levy, Sara Neuman, David Yaffe, Duchenne muscular dystrophy gene product is not identical in muscle and brain. Nature. ,vol. 337, pp. 76- 78 ,(1989) , 10.1038/337076A0
Daniel Jung, Françoise Pons, Jean J. Léger, Dominique Aunis, Alvaro Rendon, Dystrophin in central nervous system: a developmental, regional distribution and subcellular localization study. Neuroscience Letters. ,vol. 124, pp. 87- 91 ,(1991) , 10.1016/0304-3940(91)90828-H