Dp71, utrophin and beta-dystroglycan expression and distribution in PC12/L6 cell cocultures
作者： Ramses Ilarraza-Lomeli , Bulmaro Cisneros-Vega , Maria de Lourdes Cervantes-Gomez , Dominique Mornet , Cecilia Montañez
DOI: 10.1097/WNR.0B013E3282F0E42D
关键词:
摘要: Function of dystrophin Dp71 isoforms is unknown but seems related to neurite outgrowth and synapse formation. To evaluate role in myoneural synapses, we established a coculture model using PC12 cells L6 myotubes analyzed expression localization proteins, utrophin beta-dystroglycan, cells. Confocal microscopy showed Dp71d isoform nuclei, golgi-complex-like endoplasmic reticulum-like structures, whereas Dp71ab concentrates at tips cytoplasm, colocalizing with utrophin, synaptophysin acetylcholine receptors. Evidences suggest that isoform, unlike Dp71d, may take part neurite-related processes. This the first work on Dp members Dp-associated protein complex roles cell-line based coculturing system, which be useful determining associations.
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