Characterization of Dp71Δ(78-79), a novel dystrophin mutant that stimulates PC12 cell differentiation.
作者： Jorge Aragón , José Romo-Yáñez , Alejandro Martínez-Herrera , Víctor Ceja , Alvaro Rendon
DOI: 10.1111/J.1471-4159.2011.07347.X
关键词:
摘要: Dp71 has an important role in the central nervous system. To better understand function of domains neuronal differentiation, PC12 cells were stably transfected with a dystrophin mutant, Dp71Δ(78-79) , which lacks exons 78 and 79. Based on percentage bearing neurites neurite length analyses, we found that expressing (PC12-C11) differentiate more efficiently than non-transfected cells. While wild-type reach their maximum differentiation 9-12 days after initiating process, PC12-C11 4-6 days. Protein expression analysis showed down-regulation Dp71a up-regulation Dp71ab and/or Up71, β-dystroglycan neuron-specific enolase undifferentiated neural growth factor differentiated No change was observed Grb2 Up400. The subcellular localization cell periphery, there no during also throughout extensions.
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