A rapid and simple method for DNA extraction from yeasts and fungi isolated from Agave fourcroydes.
作者： Raul Tapia-Tussell , Alfonso Larqué-Saavedra , Andrés Quijano-Ramayo , Daisy Perez-Brito , Patricia Lappe
DOI: 10.1385/MB:33:1:67
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摘要: A simple and easy protocol for extracting high-quality DNA from different yeast filamentous fungal species is described. This method involves two important steps: first, the disruption of cell walls by mechanical means freezing; second, extraction, isolation, precipitation genomic DNA. The absorbance ratios (A(260)/A(280)) obtained ranged 1.6 to 2.0. main objective this procedure extract pure fungi, including those with high contents proteins, polysaccharides, other complex compounds in their walls. yield quality DNAs were suitable micro/minisatellite primer-polymerase chain reaction (MSP-PCR) fingerprinting as well sequence D1/D2 domain 26S rDNA.
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sci-hub.se 参考文章(16)
G. Cardinali, A. Bolano, A. Martini, A DNA extraction and purification method for several yeast genera Annals of Microbiology. ,vol. 51, pp. 121- 130 ,(2001)
Antonio Ippolito, Leonardo Schena, Franco Nigro, None, Detection of Phytophthora nicotianae and P. citrophthora in Citrus Roots and Soils by Nested PCR European Journal of Plant Pathology. ,vol. 108, pp. 855- 868 ,(2002) , 10.1023/A:1021208106857
D. Yarrow, Methods for the isolation, maintenance and identification of yeasts The Yeasts (Fourth Edition)#R##N#A Taxonomic Study. pp. 77- 100 ,(1998) , 10.1016/B978-044481312-1/50014-9
Hansjörg Prillinger, Orsolya Molnár, Frieda Eliskases-Lechner, Ksenija Lopandic, Phenotypic and genotypic identification of yeasts from cheese. Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology. ,vol. 75, pp. 267- 283 ,(1999) , 10.1023/A:1001889917533
Diego Libkind, Silvia Brizzio, Alejandra Ruffini, Mário Gadanho, Maria van Broock, José Paulo Sampaio, Molecular characterization of carotenogenic yeasts from aquatic environments in Patagonia, Argentina. Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology. ,vol. 84, pp. 313- 322 ,(2003) , 10.1023/A:1026058116545
R_A DeScenzo, TC Harrington, Use of (CAT)5as a DNA Fingerprinting Probe for Fungi Phytopathology. ,vol. 84, pp. 534- 540 ,(1994) , 10.1094/PHYTO-84-534
Raul Tapia-Tussell, Andres Quijano-Ramayo, Rafael Rojas-Herrera, Alfonso Larque-Saavedra, Daisy Perez-Brito, A Fast, Simple, and Reliable High-Yielding Method for DNA Extraction From Different Plant Species Molecular Biotechnology. ,vol. 31, pp. 137- 140 ,(2005) , 10.1385/MB:31:2:137
Marc-André Lachance, William T. Starmer, Herman J. Phaff, Identification of yeasts found in decaying cactus tissue Canadian Journal of Microbiology. ,vol. 34, pp. 1025- 1036 ,(1988) , 10.1139/M88-181
George S. Mahuku, A simple extraction method suitable for PCR-based analysis of plant, fungal, and bacterial DNA Plant Molecular Biology Reporter. ,vol. 22, pp. 71- 81 ,(2004) , 10.1007/BF02773351
T. Wangsomboondee, J. B. Ristaino, Optimization of Sample Size and DNA Extraction Methods to Improve PCR Detection of Different Propagules of Phytophthora infestans. Plant Disease. ,vol. 86, pp. 247- 253 ,(2002) , 10.1094/PDIS.2002.86.3.247